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[Transfer] Quantity One application ugg cardy boots
GEL EQ gel imaging guide
1. System is turned on and the computer power.
2. Click gel imaging software, enter the QUANTITY ONE interface.
3. In the FILE menu, select GEL EQ menu, enter the image acquisition interface
4. Click the LIVE / FOCUS menu, and capture images. (according to the requirements of being swept glue, gel imaging in the host panel, select UV / WHITE UV / white light). (such as transmission to be used white, put the white power on Taiwan, the white table on the floor, put glue on the white table.)
5. adjust the aperture size, click on the next IRIS OPEN / CLOSE; adjust the focus, click FOCUSE under the NEAR / FAR buttons; adjust the distance or size, click the appropriate button under ZOOM, until satisfied.
6. exposing the general time you can choose to automatically AUTO EXPOSE.
7. If the images are highlighted red dot, indicating that came to light over, shall reduce the aperture.
8. Tuxiang a good tune, click the SAVE button to save. For a save to other formats, in the FILE menu, select Export, save it as JPEG / TIFF format .
9. on the analysis and processing, see Quantity One use of handouts
Quantity One application of the lecture notes introduction
Quantity One analysis of two important functions: quantitative analysis and molecular weight determined.
Quantity One software HelpàQuick Guide quick guide to suggest how to implement a function, such as quantitative analysis (Volumes Quick Guide), Analysis of electrophoretic bands (Band Analysis), differential display analysis (Differential Display Analysis), clone count (Colony Counting Analysis ) and so on. 1,2,3 … quick guide to the steps under, according to the directions.
one. quantitative analysis
quick guide Volumes Quick Guide, this feature can be of any quantitative analysis of the selected area, this analysis can report two results: the relative concentration and absolute concentration (if the concentration of standard sample )
1. Select imaging system or open a saved file (the software can control all of the Bio-Rad imaging system, such as GelDoc, ChemiDoc, VersaDoc, GS-800, FX, such as Bio-Rad imaging system is not the intake image, the image is saved as Tiff file format, the software can also be analytical processing)
2.Zoom Box, zoom, zoom in on the image transformation observed
3.Transform, adjust the contrast of the image black and white
4.Volume Rect Tool rectangle selection, such as the U1 5.Volume Free hand Tool free brush selection area, you can outline the irregular shapes, such as U2 6.Volumn Contour Tool contours outline areas, automatically connecting the concentration concentration of the same points, such as U3
select the above 3 methods of quantitative area. Double-click the selected area
, the following window appears, define the type of sample, such as the unknown samples Unkonwn (U1, U2 … that is to be calculated sample), Background Background (B1, B2 …, software, operator concentration in this region when concentration automatically deducted) or standard sample Standard (Std1, Std2, if the sample is the concentration of standard sample, that is, quantitative criteria to be entered in the column density concentration of the sample concentration.)
7.Select Tool to select a different region
8.Print Image Print Image
9.Volume Analysis Report the results of the quantitative report, open the following window appears, select the report parameters, there are two parameters Volume and Concentration. Volume is the relative concentration, where the concentration of standard sample (Std), the software can report the absolute concentration Concentration.
II. band analysis Band Analysis
introduced the use of this feature on all the bands within the lane for molecular weight to determine the relative concentration of
1,2,3 Ibid
4, 5,6 determine the lane lane, and can be adjusted for various lane (lane is often not the actual experiment, the emergence of a variety of vertical deformation. in the software, there is a Lane Tool toolbar icon, contains more lanes editing tools. < br /> Band Tool
band editing toolkit
Lane Tool
lane editing toolkit
7.Lane Background Removal lane background, it is recommended not to
8. Detect Band, detection of bands within the lane, open the following window appears, and can be adjusted by adjusting the sensitivity to detect the sensitivity of the strip, or by human editors to increase / decrease in band. open the toolbar editing tools band Band Tool package can see more editing tools (shown above)
10.Standard enter the molecular weight standards, the software comes with a lot of Bio-Rad molecular weight standards, such as you use their own standards, into the New Standard to create a new molecular weight standards, the software can select the standard unit is the MW (protein) or BP (nucleic acid), etc. (below left). In Protein-Standard dialog box, enter the weight values ??assigned are finished, click the icon (bottom right), back to map as the standard sample file, click the lane, the software will automatically lose the weight values ??and the lanes correspond to the bands.
assignment icon:
tell the software which one is the molecular weight standards
Enter the sample weight values ??lane
10.Band Attribute band property, after the completion of step 9, the software is automatically calculated molecular weight of other unknown bands, open the band attribute to select the report parameters Here we have chosen Molecular Weight, images can be seen on the molecular weight of all the bands (red tag), the standard molecular weight markers in blue.
11.Match, skip do
12.Print Image Print Image
13.All Lane Report report on the results of all lane striping. open the window, select the report parameters, such as Mol.Wt. (molecular weight, left hand side below), the software will report the results (below right ), lower right leg in the report there is a report output tool, click on the report results can be output into a TXT text format or output to the clipboard, you can paste it into EXCEL, WORD and other documents under.
report the results of the output tools < br /> If you have questions, please contact the company of Chengdu Science and Technology Park.
Tel: 13880582991 (Kinson)
13980888640 (fee Ka)
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